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th UNC5A may interfere with UNC5A binding to apoptosis-promoting factors, such as MAGED1. Consequently, FANCC may act as a cellular sensor of UNC5A-mediated apoptotic cues and prevent or delay apoptosis depending on the tissue or cellular context. Acknowledgments We are grateful to Dr. Maureen Hoatlin for the 8F3 monoclonal anti-FANCC antibodies and to Dr Tessier-Lavigne for providing the pSecMyc-
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s . Total IgE was assessed using the ImmunoCAP System and the cutoff was 2 kU/ L. RT-PCR and Quantitative Real-time PCR Total RNA was isolated from white blood cells using the QIAmp RNA Blood Mini Kit supplemented with RNase-free DNase and stored at minus 80uC. For reverse transcription of RNA we used 300 ng of total RNA in a final volume of 30 ml and added adequate amounts of TaqMan Reverse Trans
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