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ering 65: 382388. 44. Diaz AA, Tomba E, Lennarson R, Richard R, Bagajewicz MJ, et al. Prediction of protein solubility in Escherichia coli using logistic regression. Biotechnology and Bioengineering 105: 374383. 45. Shirano Y, Shibata D Low temperature cultivation of Escherichia coli carrying a rice lipoxygenase L-2 cDNA produces a soluble and active enzyme at a high level. FEBS Letters 271: 12813
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ware. The trajectories of the determined centroids during the 8-hour period were examined to evaluate the migration phenotypes as discussed below. The migration rate was calculated by dividing the summed distances between the consecutive centroid positions of the track by the time, and the directional persistence was obtained as the net distance between the first and last positions of centroids di
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